Manhart Lab
at Temple University
Department of Chemistry
We develop mechanistic models to understand the enzymology of nucleases involved in DNA repair. Nucleases hydrolyze the phosphodiester backbone of nucleic acid molecules and are involved in cell cycle quality control mechanisms. They are a diverse family of enzymes and potential drug targets since defects in nucleases are associated with human diseases ranging from cancer, immunodeficiency, to amyloid-linked diseases. They have also been co-opted for genetic engineering, therapeutics, and disease diagnostic technologies (CRISPR Cas9) which are also avenues of interest for our lab.
Work in the Manhart lab aims to elucidate DNA repair mechanisms and regulation. Our work contributes to models of human disease, particularly Lynch syndrome and trinucleotide repeat instability.
Our work provides a foundation for understanding fundamental cellular pathways and developing new diagnostic tools, identifying new therapeutic targets, and developing technology to improve nucleases used in clinical and biotechnological applications.
We use combinations of biochemistry, biophysics, chemistry, molecular biology, and genetics to understand this diverse family of enzymes.
Work in the Manhart lab aims to elucidate DNA repair mechanisms and regulation. Our work contributes to models of human disease, particularly Lynch syndrome and trinucleotide repeat instability.
Our work provides a foundation for understanding fundamental cellular pathways and developing new diagnostic tools, identifying new therapeutic targets, and developing technology to improve nucleases used in clinical and biotechnological applications.
We use combinations of biochemistry, biophysics, chemistry, molecular biology, and genetics to understand this diverse family of enzymes.
Sponsors
Our research is supported by the following:
Manhart Lab News4/12/22: Congratulations to Manhart Lab graduate student Isabella Rosa on officially becoming a PhD candidate!
1/14/22: Huge welcome to our newest lab members: Bryce Collingwood and Jonathan Piscitelli! 1/14/21: Congratulations to Manhart Lab graduate student Scott Witte on officially becoming a PhD candidate! 10/28/20: Congratulations to Manhart Lab graduate student Isabella Rosa on her literature seminar entitled, "Conjunction-Junction, RuvC function". 08/21/20: Congratulations to Manhart Lab graduate student Isabella Rosa on passing all of her cumulative exams! 07/26/20: Manhart Lab group meetings are now on Fridays 9-11am over Zoom. Our meetings are open to prospective students! Students in the lab present a mix of their current research, techniques in the field, and new developments in literature. Inquires regarding group meetings can be made at [email protected]. 06/09/20: The Manhart lab is back in action (whilst still using proper PPE and maintaining social distancing tactics)! Please remember to wash your hands, socially distance, and to wear masks! |
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